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CYP17A1 antibody

Anti-CYP17A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56294
Anti-CYP17A1 antibody used in Western Blot (WB). GTX56294
Anti-CYP17A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX56294

Cat. No. GTX56294

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CYP17A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of CYP17A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 17 subfamily A member 1 , CPT7 , CYP17 , P450C17 , S17AH

Cellular Localization

Membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP17A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56294

GTX56294 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX56294 CYP17A1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-CYP17A1 antibody used in Western Blot (WB). GTX56294

GTX56294 WB Image

WB analysis of HeLa (A), NIH3T3 (B), H9C2 (C), SW13 (D), ES2 (E) whole cell lysates using GTX56294 CYP17A1 antibody.

Anti-CYP17A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX56294

GTX56294 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX56294 CYP17A1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Feng X et al. Mol Cell Endocrinol 2021; 519 : 111039 Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
Application : ICC/IF
Reactivity : Human
Kong ZL et al. Nutrients 2018; 10 (10) Effect of Cistanche Tubulosa Extracts on Male Reproductive Function in Streptozotocin-Nicotinamide-Induced Diabetic Rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399