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CYP1A1 antibody [6G5]

Anti-CYP1A1 antibody [6G5] used in Western Blot (WB). GTX60543
Anti-CYP1A1 antibody [6G5] used in IHC (Paraffin sections) (IHC-P). GTX60543
Anti-CYP1A1 antibody [6G5] used in ELISA (ELISA). GTX60543
Anti-CYP1A1 antibody [6G5] used in IHC (Paraffin sections) (IHC-P). GTX60543

Cat. No. GTX60543

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G5

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all CYP1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CYP1A1(AA : 203-461) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 1 subfamily A member 1 , AHH , AHRR , CP11 , CYP1 , CYPIA1 , P1-450 , P450-C , P450DX

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion inner membrane,Cytoplasm

Background

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP1A1 antibody [6G5] used in Western Blot (WB). GTX60543

GTX60543 WB Image

WB analysis of human CYP1A1 (AA: 203-461) recombinant protein using GTX60543 CYP1A1 antibody [6G5].

Anti-CYP1A1 antibody [6G5] used in IHC (Paraffin sections) (IHC-P). GTX60543

GTX60543 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60543 CYP1A1 antibody [6G5].

Anti-CYP1A1 antibody [6G5] used in ELISA (ELISA). GTX60543

GTX60543 ELISA Image

ELISA analysis of antigen using GTX60543 CYP1A1 antibody [6G5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-CYP1A1 antibody [6G5] used in IHC (Paraffin sections) (IHC-P). GTX60543

GTX60543 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60543 CYP1A1 antibody [6G5].

REFERENCE

Application Reference

Wei KL et al. Toxicology 2022; : 153118 Sorafenib is an antagonist of the aryl hydrocarbon receptor.
Vazquez-Gomez G et al. Toxicol Lett 2018; 289 : 54-62 Benzo[a]pyrene activates an AhR/Src/ERK axis that contributes to CYP1A1 induction and stable DNA adducts formation in lung cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409