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CYP2C8 antibody

Anti-CYP2C8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113666
Anti-CYP2C8 antibody used in Western Blot (WB). GTX113666
Anti-CYP2C8 antibody used in IHC (Paraffin sections) (IHC-P). GTX113666

Cat. No. GTX113666

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CYP2C8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP2C8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723131

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 2 subfamily C member 8 , CPC8 , CYP2C8DM , CYPIIC8 , MP-12/MP-20

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CYP2C8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113666

GTX113666 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CYP2C8(GTX113666) antibody at 1:500 dilution.

Anti-CYP2C8 antibody used in Western Blot (WB). GTX113666

GTX113666 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP2C8 antibody (GTX113666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP2C8 antibody used in IHC (Paraffin sections) (IHC-P). GTX113666

GTX113666 IHC-P Image

CYP2C8 antibody detects CYP2C8 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
CYP2C8 antibody (GTX113666) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Qiu YE et al. Prostaglandins Other Lipid Mediat 2018; 136 : 43357 Increased epoxyeicosatrienoic acids may be part of a protective mechanism in human ulcerative colitis, with increased CYP2J2 and reduced soluble epoxide hydrolase expression.
Application : WB
Reactivity : Human
Song G et al. Drug Metab Dispos 2017; Determination of Human Hepatic CYP2C8 and CYP1A2 Age-Dependent Expression to Support Human Health Risk Assessment for Early Ages.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169