CYP3A4 antibody [3H8]

Cat. No. GTX60577

Reference 6

Host	Mouse
Clonality	Monoclonal
Clone Name	3H8
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA
Reactivity	Human
Package	100 μg ($399)

See all CYP3A4 products

View product citations for antibody GTX60577 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
IHC-Fr	Assay dependent
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CYP3A4 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 3 subfamily A member 4 , CP33 , CP34 , CYP3A , CYP3A3 , CYPIIIA3 , CYPIIIA4 , HLP , NF-25 , P450C3 , P450PCN1

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]

Database

Gene ID: 1576 CYP3A4
UniProt: P08684 CYP3A4

Research Area

DATA IMAGES

GTX60577 WB Image

WB analysis of HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate using GTX60577 CYP3A4 antibody [3H8].

GTX60577 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60577 CYP3A4 antibody [3H8].

GTX60577 IHC-P Image

IHC-P analysis of human brain tissue using GTX60577 CYP3A4 antibody [3H8].

GTX60577 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60577 CYP3A4 antibody [3H8].
Green : CYP3A4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60577 ELISA Image

ELISA analysis of antigen using GTX60577 CYP3A4 antibody [3H8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng