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Casein Kinase 2 beta (phospho Ser209) antibody

Orthogonal
Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861
Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

Cat. No. GTX12861

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
50 μl ($399)
See all Casein Kinase 2 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Product Note

Does not cross react with Casein Kinase 2 alpha [pS₃₆] (mouse), [pS₃₆₂], [pS₃₇₀], or Casein Kinase 2 beta pS₂].

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human CK2â that contains serine 209. The sequence is conserved in mouse, rat, chicken, pig and rabbit.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

casein kinase 2 beta , CK2B , CK2N , CSK2B , Ckb1 , Ckb2 , G5A

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

GTX12861 WB Image

WB analysis of whole cell extract (30ug) of Jurkat (Lane 1), K562 (Lane 2), KARPAS 299 (Lane 3), HEL 92.1.7 (Lane 4), THP1 (Lane 5), A431 (Lane 6) and A549 (Lane 7) using GTX12861 Casein Kinase 2 beta (phospho Ser209) antibody.
Dilution : 1:250

Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

GTX12861 WB Image

Orthogonal

WB analysis of HeLa cells using GTX12861 Casein Kinase 2 beta (phospho Ser209) antibody.
The primary antibody was prior incubated with different peptide (Lane 1~4).
The membrane treated with lambda phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399