GeneTex
United States (US)

Caspase 1 (active) recombinant protein

Cat No. GTX65327

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

Protein activity determined to be 20000 units/mg. One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Calculated MW

large (20 kD) and small (10 kD) subunits. kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase1

Background

Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.

Research Area