Protein activity determined to be > 5000 units/mg. One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
large (20 kD) and small (10 kD) subunits kDa. ( Note
Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.
Purity was assessed by SDS-PAGE and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ICE, IL1BC, P45, Caspase 1, Caspase1, Active Human Caspase-1, Active Caspase-1, Caspase-1 Enzyme, Recombinant caspase-1, human caspase-1, human caspases, active caspases
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.