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Caspase 10 antibody

Anti-Caspase 10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113148
Anti-Caspase 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX113148
Anti-Caspase 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX113148
Anti-Caspase 10 antibody used in Western Blot (WB). GTX113148

Cat. No. GTX113148

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Caspase 10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174945

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 10 , ALPS2 , FLICE2 , MCH4

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113148

GTX113148 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 10(GTX113148) antibody at 1:500 dilution.

Anti-Caspase 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX113148

GTX113148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Caspase 10(GTX113148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX113148

GTX113148 IHC-P Image

Caspase 10 antibody detects Caspase 10 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Caspase 10 antibody (GTX113148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 10 antibody used in Western Blot (WB). GTX113148

GTX113148 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: NCI-H929
7.5% SDS PAGE
GTX113148 diluted at 1:1000

REFERENCE

Application Reference

Xu SJ et al. Apoptosis 2018; (Epub) Flavonoids of Rosa roxburghii Tratt offers protection against radiation induced apoptosis and inflammation in mouse thymus.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169