GeneTex
United States (US)

Caspase-3 Fluorometric Assay Kit

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Cat No. GTX85557

PRODUCT

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.

‧ Detection method- Flow cytometry (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
DEVD-AFC (1 mM)
DTT (1 M)

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase-3 Assay, Caspase 3 Activity Assay, Caspase substrate kit, Apoptosis Assay, Caspase-3 Substrate, Caspase-3 Activity Assay, Caspase3 Substrate, Caspase 3 Substrate, Caspase3 Assay, Caspase3 Activity Assay, Caspase 3 Assay
DATA IMAGES
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GTX85557 Image

Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

GTX85557_CaspaseSubstrateProtocol_19012407_472.jpg

GTX85557 Image


REFERENCE
Package List Price ($)
$ 369