GeneTex
United States (US)

Caspase 3 (active) recombinant protein

Cat No. GTX65330

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Rat
Species Rat
APPLICATION

Application Note

Protein activity determined to be 300000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Calculated MW

of large (17 kD) and small (11 kD) subunits kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.

Antigen Species

Rat

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase3

Background

Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at GeneTex for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA or Ac-DEVD-AFC.

Research Area