Application Note
Protein activity determined to be 300,000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Calculated MW
large (17 kD) and small (11 kD) subunits kDa. (
Note )
Form
Lyophilized powder
Storage
Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.
Antigen Species
Rat
Expression System
E. coli
Purification
Purity was assessed by SDS-PAGE and by HPLC.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
A830040C14Rik, AC-3, Apopain, CC3, CPP32, Caspase-3, Lice, Yama, mldy, AC 3, AC3, Caspase3
Background
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at GeneTex for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA or Ac-DEVD-AFC.
Research Area
