GeneTex
United States (US)

Caspase 4 recombinant protein

Cat No. GTX65034

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein activity determined to be > 5000 units/mg. One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX, ICH 2, ICEREL II, Caspase 4, ICH2, ICERELII, Caspase4

Background

Caspase-4 (also know as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-4 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-4 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. The recombinant active human caspase-4 was expressed in E. coli. The active caspase-4 is routinely tested at GeneTex for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA or Ac-WEHD-AFC.

Research Area

Package List Price ($)
$ 229