GeneTex
United States (US)

Caspase 6 recombinant protein

Caspase-6-recombinant-protein_WB_GTX65038-1_18121409_305.jpg

Cat No. GTX65038

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein activity determined to be > 13000 units/mg. One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Calculated MW

large (18 kDa) and small (11 kDa) kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 6,Mch2,Casp6

Background

Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli. The active caspase-6 is routinely tested at GeneTex for its ability to enzymatically cleave these two substrates Ac-VEID-pNA or Ac-VEID-AFC.

Research Area

DATA IMAGES
Caspase-6-recombinant-protein_WB_GTX65038-1_18121409_305.jpg

GTX65038 WB Image

Western blot analysis of Caspase-6 recombinant protein using GTX65037

Package List Price ($)
$ 229