GeneTex
United States (US)

Caspase-8 (Active) recombinant protein

Cat No. GTX65333

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

Protein activity determined to be 5000 units/mg. One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to 1 unit per μl in PBS containing 15% glycerol. Store at -80ºC.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase8, CASP8, Caspase 8

Background

Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis.

Research Area