GeneTex
United States (US)

Cathepsin D (Active) protein

Cat No. GTX65066

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein activity determined to be 365 units/mg. >300 units/mg protein. One unit is defined as the amount of enzyme that digests hemoglobin releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37ºC. Substrate: acid denatured hemoglobin (0.2% in reaction mixture). Buffer: 100 mM formate. pH 3.3.
PROPERTIES

Form

Lyophilized powder

Buffer

2 mM sodium phosphate, pH 6.5

Storage

Reconstitute the vial in water to a concentration of 0.1-1 μg/μl. Aliquot and refreeze at -80ºC immediately for future use.

Antigen Species

Human

Expression System

Human liver

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTSB, human), Cathepsin D (active

Background

Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node negative and node positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer’s plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers.

Research Area