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Cdh2 antibody

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962
Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962
Anti-Cdh2 antibody used in Western Blot (WB). GTX125962
Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962

Cat. No. GTX125962

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Cdh2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

zebrafish brain

Predict Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish Cdh2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885620

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2, type 1, N-cadherin (neuronal) , bib , glo , lyr , ncad , pac , wu:fb47h04

Cellular Localization

Cell membrane

Background

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. N-cadherin may be involved in neuronal recognition mechanism. Controls neural tube morphogenesis. Required to allow mid- and hindbrain neurons to reach and maintain their appropriate positions within the neural tube by restricting neuronal motility within the embryonic mid- and hindbrain.

Database

Research Area

DATA IMAGES

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962

GTX125962 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cdh2 stained by Cdh2 antibody (GTX125962) diluted at 1:200.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962

GTX125962 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cdh2 stained by Cdh2 antibody (GTX125962) diluted at 1:200.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Cdh2 antibody used in Western Blot (WB). GTX125962

GTX125962 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX125962 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125962

GTX125962 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein at on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cdh2 stained by Cdh2 antibody (GTX125962) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

REFERENCE

Application Reference

Ferre-Fern?ndez JJ et al. Hum Genomics 2022; 16 (1) : 49 CRISPR-Cas9-mediated functional dissection of the foxc1 genomic region in zebrafish identifies critical conserved cis-regulatory elements.
Application : IHC-Wm
Reactivity : Zebrafish
Seese SE et al. Dev Dyn 2021; 250 (8) : 1056-1073 Genetic disruption of zebrafish mab21l1 reveals a conserved role in eye development and affected pathways.
Application : IHC-P
Reactivity : Zebrafish
Brandt ZJ et al. Development 2020; 147 (12) Core Hippo pathway components act as a brake on Yap and Taz in the development and maintenance of the biliary network.
Application : IHC-Wm
Reactivity : Zebrafish
Kathryn E. Hendee et al. bioRxiv 2019; (Epub) notum1, acting downstream of pitx2, is essential for proper eye and craniofacial development.
Application : IHC-P
Reactivity : Zebrafish
Warga RM et al. Genetics 2018; 210 (4) : 1391-1409 Probing Cadherin Interactions in Zebrafish with E- and N-Cadherin Missense Mutants.
Application : IHC-Wm
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169