Centrin 1 antibody [N1C3]

Name: Centrin 1 antibody [N1C3]
Brand: GeneTex
SKU: GTX114316
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Centrin 1 antibody [N1C3] used in Immunoprecipitation (IP). GTX114316

Anti-Centrin 1 antibody [N1C3] used in Western Blot (WB). GTX114316

Anti-Centrin 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114316

Cat. No. GTX114316

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Bovine
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX114316 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Jurkat , 293T , A431

Predict Reactivity

Mouse, Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619101

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrin 1 , CEN1 , CETN

Cellular Localization

Centrosome

Background

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq]

Database

Gene ID: 1068 CETN1
UniProt: Q12798 CETN1

Research Area

DATA IMAGES

GTX114316 IP Image

Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 μg of Centrin-1 antibody [N1C3] (GTX114316).
Western blot analysis was performed using Centrin-1 antibody [N1C3] (GTX114316) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114316 WB Image

Centrin 1 antibody detects Centrin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody (GTX114316) diluted at a dilution of 1:1000.

GTX114316 ICC/IF Image

Centrin 1 antibody [N1C3] detects Centrin 1 protein at centrosome by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Centrin 1 stained by Centrin 1 antibody [N1C3] (GTX114316) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114316 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
12% SDS PAGE
Centrin-1 antibody
GTX114316 diluted at 1:1000

REFERENCE

Application Reference

Ito S et al. J Reprod Dev 2023; 69 (1) : 18-24 Immunohistochemical identification of epithelial cell types in the isthmus of bovine oviduct: Comparison with the ampulla.

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REVIEW