GeneTex

  • Country / Location Selection

United States (US)

Clathrin heavy chain antibody [X22]

KO/KD
Anti-Clathrin heavy chain antibody [X22] used in IHC (Paraffin sections) (IHC-P). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in IHC (Paraffin sections) (IHC-P). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in Western Blot (WB). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731
Anti-Clathrin heavy chain antibody [X22] used in Western Blot (WB). GTX22731

Cat. No. GTX22731

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 X22

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC, IM, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Hamster, Primate
Package
100 μl ($399)
See all Clathrin heavy chain products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:500
ICC/IF 1:1,000
IHC-P 1:100
IHC-Fr Assay dependent
FACS 1/200
IP Assay dependent
IHC Assay dependent
IM Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Product Note

Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human brain clathrin heavy chain.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_369614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

clathrin heavy chain , CHC , CHC17 , CLH-17 , CLTCL2 , Hc , MRD56

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Clathrin heavy chain antibody [X22] used in IHC (Paraffin sections) (IHC-P). GTX22731

GTX22731 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Clathrin heavy chain antibody [X22] used in IHC (Paraffin sections) (IHC-P). GTX22731

GTX22731 IHC-P Image

IHC-P analysis of human colon tissue using GTX22731 Clathrin heavy chain antibody [X22].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731

GTX22731 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Clathrin heavy chain antibody [X22] used in Western Blot (WB). GTX22731

GTX22731 WB Image

WB analysis of 25 ug of human brain lysates using GTX22731 Clathrin heavy chain antibody [X22].
Dilution : 1:300

Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731

GTX22731 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Clathrin heavy chain antibody [X22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22731

GTX22731 ICC/IF Image

ICC/IF analysis of NCI-H460 Cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Clathrin heavy chain antibody [X22] used in Western Blot (WB). GTX22731

GTX22731 WB Image

KO/KD

The data was published in the journal Sci Rep in 2019.PMID: 30787373

REFERENCE

Application Reference

Chao PK et al. Sci Rep 2019; 9 (1) : 2405 Convallatoxin enhance the ligand-induced mu-opioid receptor endocytosis and attenuate * antinociceptive tolerance in mice.
Application : WB
Reactivity : Mouse
Chao PK et al. bioRxiv 2017; (Epub) Drug discovery to counteract antinociceptive tolerance with mu-opioid receptor endocytosis.
Application : IHC-Fr
Reactivity : Mouse
Smith TH et al. J Biol Chem 2016; (Epub) Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of β-arrestins.
Application : ICC/IF
Reactivity : Human
Parks EE et al. J Biol Chem 2014; Cell Surface Epidermal Growth Factor Receptors Increase Src and c-Cbl Activity and Receptor Ubiquitylation.
Application : ICC/IF
Reactivity : Human
Coradeghini R et al. Toxicol Lett 2013; 217 (3) : 205-16 Size-dependent toxicity and cell interaction mechanisms of gold nanoparticles on mouse fibroblasts.
Application : WB
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Clathrin heavy chain antibody [X22] (GTX22731). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399