APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888056
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cofilin 1 , CFL , HEL-S-15 , cofilin
Cellular Localization
Nucleus matrix , Cytoplasm , cytoskeleton
Background
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/templates/default/js/lightbox/images/loading.gif "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
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GTX628804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 IHC-P Image
Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody [GT567] (GTX628804) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 ICC/IF Image
Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for Cofilin 1 antibody [GT567] (GTX628804). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cofilin 1 antibody [GT567] (GTX628804). Be the first to share your experience with this product.
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_w_23061202_779.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_IHC_w_23061202_146.webp "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_R_w_23061202_195.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_M_w_23061202_861.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20150807_IFA_w_23061202_961.webp "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_w_23061202_779.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_IHC_w_23061202_146.webp "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_R_w_23061202_195.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_M_w_23061202_861.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20150807_IFA_w_23061202_961.webp "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")