Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 - 1:10000 |
| Assay dependent |
| 1:50 - 1:200 |
| 1:100 |
| 1:5000 - 1:50000 |
|
Note :
WB
This preparation results in a native conformation of the protein. Recommend not adding 2-ME or DTT in lysis buffer and not boiling the sample.
|
Not tested in other applications.
Calculated MW
Product Note
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Form
Liquid
Buffer
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Purification
Purified by antigen-affinity chromatography.
This product was prepared by immunoaffinity chromatography on immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove unwanted specificities.
Conjugation
Unconjugated
RRID
AB_384293
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4
Background
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
Database
Research Area
