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Collagen IV antibody [COL-94]

Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311
Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

Cat. No. GTX26311

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 COL-94

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, Dot, ELISA, EM

Reactivity

 Human
Package
100 μl ($509)
See all Collagen IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:500
IHC-Fr Assay dependent
Dot Assay dependent
ELISA Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Product Note

Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.
We do not recommend use of this product for Rat,Rabbit,Goat,Sheep,Cat,Chicken samples.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

human collagen type IV

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_368873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type IV alpha 1 chain , BSVD , RATOR

Background

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

Database

Research Area

DATA IMAGES

Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

GTX26311 IHC-P Image

IHC-P analysis of human placenta sections using GTX26311 Collagen IV[COL-94] antibody at 1:500.

Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

GTX26311 IHC-P Image

IHC-P analysis of human tongue tissue using GTX26311 Collagen IV antibody [COL-94] at 1:500.

REFERENCE

Application Reference

Baker JHE et al. Clin Exp Metastasis 2018; 35 (7) : 691-705 Heterogeneous distribution of * in HER2-positive xenografts and metastases: role of the tumor microenvironment.
Application : IHC-Fr
Reactivity : Human
Huang YH et al. Exp Biol Med (Maywood) 2017; : 1.54E+15 MicroRNA-29a mitigation of endoplasmic reticulum and autophagy aberrance counteracts in obstructive jaundice-induced fibrosis in mice.
Application : WB
Reactivity : Rat
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Application : WB
Reactivity : Human
McLane JS et al. Cancer Microenviron 2016; (Epub) Stiffened Extracellular Matrix and Signaling from Stromal Fibroblasts via Osteoprotegerin Regulate Tumor Cell Invasion in a 3-D Tumor in Situ Model.
Application : ICC/IF
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 509