APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 367 (R-A-Sp-S-R).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11164238
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AVSD3,CMDR,CX43,EKVP,GJA1,GJAL,HLHS1,HSS,ODDD,PPKCA,gap junction protein alpha 1,Connexin 43
Cellular Localization
Cell membrane,Cell junction,Gap junction,Endoplasmic reticulum
Background
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
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GTX79102 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX79102 Connexin 43 (phospho Ser368) antibody.
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GTX79102 WB Image
WB analysis of HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11), and 293 (Line 3, 6, 9 and 12) cells untreated or treated with PMA (1μM 30min) lysates using GTX79102 Connexin 43 (phospho Ser368) antibody.
Lane 1 - 6 : Connexin 43 antibody
Lane 7 - 12 : GTX79102 Connexin 43 (phospho Ser368) antibody
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REFERENCE
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REVIEW
There are currently no reviews for Connexin 43 (phospho Ser368) antibody (GTX79102). Be the first to share your experience with this product.
