Japanese encephalitis virus Core protein C antibody

Name: Japanese encephalitis virus Core protein C antibody
Brand: GeneTex
SKU: GTX131368
Price: 399.00 USD
Rating: 3 (2 reviews)

Anti-Japanese encephalitis virus Core protein C antibody used in Western Blot (WB). GTX131368

Anti-Japanese encephalitis virus Core protein C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131368

Cat. No. GTX131368

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Japanese encephalitis virus
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX131368 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:3000

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

JEV infected BHK-21 cells

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Core protein C (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886468

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Core , JEV Core

Background

The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions.

Research Area

DATA IMAGES

GTX131368 WB Image

Core protein C (JEV) antibody detects Core protein C (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Core protein C (JEV) antibody (GTX131368) at a dilution of 1:3000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX131368 ICC/IF Image

Core protein C (Japanese encephalitis virus) antibody detects Core protein C (Japanese encephalitis virus) by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol.
Green: Core protein C (Japanese encephalitis virus) stained by core protein C (Japanese encephalitis virus) antibody (GTX131368) diluted at 1:3000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.

Sapna Sehrawat et al. J Virol 2021; Valosin-containing protein/p97 plays critical roles in the Japanese encephalitis virus life cycle.

Application : WB, ICC/IF

Sarkar R et al. J Gen Virol 2020; (Epub) Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets.

Application : WB, ICC/IF

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