Coxsackie Adenovirus Receptor antibody

Name: Coxsackie Adenovirus Receptor antibody
Brand: GeneTex
SKU: GTX118382
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Coxsackie Adenovirus Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118382

Anti-Coxsackie Adenovirus Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX118382

Anti-Coxsackie Adenovirus Receptor antibody used in Immunoprecipitation (IP). GTX118382

Anti-Coxsackie Adenovirus Receptor antibody used in Western Blot (WB). GTX118382

Cat. No. GTX118382

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all Coxsackie Adenovirus Receptor products

View product citations for antibody GTX118382 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Mouse, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coxsackie Adenovirus Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175493

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CXADR Ig-like cell adhesion molecule , CAR , CAR4/6 , HCAR

Cellular Localization

Isoform 1: Cell membrane , Cell junction , tight junction , adherens junction , Basolateral cell membrane , Isoform 2: Cell membrane , Basolateral cell membrane , Isoform 3: Secreted , Isoform 4: Secreted , Isoform 5: Secreted

Background

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq]

Database

Gene ID: 1525 CXADR
UniProt: P78310 CXADR

Research Area

DATA IMAGES

GTX118382 ICC/IF Image

Coxsackie Adenovirus Receptor antibody detects Coxsackie Adenovirus Receptor protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Coxsackie Adenovirus Receptor stained by Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX118382 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR(GTX118382) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX118382 IP Image

Immunoprecipitation of CAR protein from HeLa whole cell extracts using 5 μg of CAR antibody (GTX118382).
Western blot analysis was performed using CAR antibody (GTX118382).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX118382 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Naito H et al. Environ Health Prev Med 2016; (Epub) Importance of detoxifying enzymes in differentiating fibrotic development between SHRSP5/Dmcr and SHRSP rats.

Application : WB

Reactivity : Rat

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REVIEW