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Cpn10 antibody

Anti-Cpn10 antibody used in Western Blot (WB). GTX13528

Cat. No. GTX13528

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, IHC, RIA

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig, Xenopus laevis
Package
100 μl ($399)
See all Cpn10 products

APPLICATION

Application Note

IHC: Use at a dilution of 1/400. IP: Use at a dilution of 1/100. RIA: Use at a dilution of 1/100000. WB (colorimetric): Use at a dilution of 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). WB (ELC): Use at a dilution of 1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

11 kDa. ( Note )

Positive Control

HeLa Heat Shocked Cell LysateRecombinant Human Chaperonin 10 Protein

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 50% glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide: RDGDILGKYVD conjugated to KLH, corresponding to amino acids 91-101 of Human Cpn10.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family E (Hsp10) member 1 , CPN10 , EPF , GROES , HSP10

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-Cpn10 antibody used in Western Blot (WB). GTX13528

GTX13528 WB Image

Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Cpn10 (Hsp10) pAb.

REFERENCE

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Package List Price ($)
$ 399