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Creatine kinase (brain) antibody [N1C1]

Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759

Cat. No. GTX101759

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Creatine kinase (brain) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240631

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase B , B-CK , BCK , CKBB , CPK-B , HEL-211 , HEL-S-29

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759

GTX101759 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX101759 diluted at 1:1000

Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759

GTX101759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (brain) antibody [N1C1] (GTX101759) at 1:300 dilution.

Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759

GTX101759 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759

GTX101759 ICC/IF Image

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759

GTX101759 IHC-P Image

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Creatine kinase (brain) stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759

GTX101759 ICC/IF Image

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) protein stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759

GTX101759 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759

GTX101759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Creatine kinase (brain) antibody(GTX101759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lin ST et al. J Proteomics 2012; 75 (18) : 5822-47 proteomic analysis of proteins responsible for the development of * resistance in human uterine cancer cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169