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Cyclin B1 antibody

Orthogonal
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

Cat. No. GTX100911

Reference 40
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, African green monkey
Package
100 μl ($399),
25 μl ($169)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949886

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm , Nucleus , Centrosome

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Sample (50 μg of HeLa whole cell lysate)
GTX100911 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100911

GTX100911 IHC-P Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Cyclin B1 antibody (GTX100911) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Cyclin B1 antibody detects Cyclin B1 protein by Western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract |
D. 1 μg 293T whole cell lysate/extract
10 % SDS-PAGE
Cyclin B1 antibody (GTX100911) dilution: 1:3000

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Sample (30 ug of whole cell lysate)
A: U2OS
B: U2OS treated with 100ng/ml Nocodazole for 16hr
10% SDS PAGE
GTX100911 diluted at 1:1000

Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911

GTX100911 ICC/IF Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911

GTX100911 ICC/IF Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2012. PMID: 22283874

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Orthogonal

The data was published in the journal PLoS One in 2014. PMID: 25105411

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

The data was published in the journal World J Surg Oncol in 2018. PMID: 29466986

REFERENCE

Application Reference

Coomar S et al. Mol Cancer Res 2023; 21 (8) : 768-778 Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation.
S Coomar et al. Mol Cancer Res 2023; Overlaid transcriptional and proteome analyses identify mitotic kinesins as important 2 targets of arylsulfonamide-mediated RBM39 degradation
Application : WB
Reactivity : Human
Zhang Y et al. Cell Death Discov 2023; 9 (1) : 82
Application : WB
Gupta RK et al. EMBO J 2022; 41 (22) : e111653 Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral?spread.
Application : WB
Reactivity : African green monkey
Liu GY et al. EMBO J 2022; 41 (15) : e110472 Precise control of microtubule disassembly in living cells.
Show More +
Submit a Reference

REVIEW

Cyclin B1 antibody Cat. No. GTX100911

Rating
( Average 4.5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 6 users reviews)
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 24-Oct-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3 cells, OECM-1 cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 07-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
Lane 1 : Control
Lane 2 : EGFR (CPT) : ZH2 (CPT) 20:0
Lane 3 : EGFR (CPT) : ZH2 (CPT) 15:5
Lane 4 : EGFR (CPT) : ZH2 (CPT) 10:10
Lane 5 : EGFR (CPT) : ZH2 (CPT) 5:15
Lane 6 : EGFR (CPT) : ZH2 (CPT) 0:20
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 24-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 17-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-5 cells, CL1-0 cells, A549 cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : T84 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Show More +
Submit a Review
Application Reference
Coomar S et al. Mol Cancer Res 2023; 21 (8):768-778 Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation.
S Coomar et al. Mol Cancer Res 2023; Overlaid transcriptional and proteome analyses identify mitotic kinesins as important 2 targets of arylsulfonamide-mediated RBM39 degradation
Application : WB
Reactivity : Human
Zhang Y et al. Cell Death Discov 2023; 9 (1):82
Application : WB
Gupta RK et al. EMBO J 2022; 41 (22):e111653 Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral?spread.
Application : WB
Reactivity : African green monkey
Liu GY et al. EMBO J 2022; 41 (15):e110472 Precise control of microtubule disassembly in living cells.
Xiaobo Wang et al. J Radiat Res 2022; 63 (3):331-341 Overcoming radio-resistance in esophageal squamous cell carcinoma via hypermethylation of PIK3C3 promoter region mediated by KDM5B loss.
Application : WB
Reactivity : Human
Shih-Hang Tai et al. Transl Cancer Res 2021; 10 (9):3906-3920 Cinnamophilin enhances temozolomide-induced cytotoxicity against malignant glioma: the roles of ROS and cell cycle arrest.
Application : WB
Reactivity : Human
Terasaki M et al. Nutr Cancer 2021;:1-16 A Marine Carotenoid of Fucoxanthinol Accelerates the Growth of Human Pancreatic Cancer PANC-1 Cells.
Terasaki M et al. Cancer Genomics Proteomics 2021; 18 (2):133-146 A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell.
Application : WB
Reactivity : Mouse
Lee W.J. et al. JournalJournal of Food and Drug Analysis 2021; 29 (1):113-127 Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects.
Coomar S. et al. bioRxiv 2021; (Epub) Arylsulfonamide mediated RBM39 degradation causes aberrant splicing of mitotic kinesins.
Application : WB
Reactivity : Human
Kuo TT et al. ACS Omega 2020; 5 (49):31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Chen SY et al. Int J Mol Med 2019; 44 (5):1687-1696 Piperlongumine induces autophagy in biliary cancer cells via?reactive oxygen species-activated Erk signaling pathway.
Application : WB
Reactivity : Human
Lin JH et al. Stem Cells International 2019; 2019:Article ID 7606238 Palmitic Acid Methyl Ester Induces G2/M Arrest in Human Bone Marrow-Derived Mesenchymal Stem Cells via the p53/p21 Pathway.
Application : WB
Reactivity : Human
Ghousein A et al. Liver Int 2019; (Epub) miR-4510 blocks hepatocellular carcinoma development through RAF1 targeting and RAS/RAF/MEK/ERK signalling inactivation.
Application : WB
Reactivity : Human
Chen CC et al. Transl Res 2019; (Epub) Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice.
Application : WB
Reactivity : Mouse
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Thermal cycling-hyperthermia in combination with polyphenols, epigallocatechin gallate and chlorogenic acid, exerts synergistic anticancer effect against human pancreatic cancer PANC-1 cells.
Application : WB
Reactivity : Human
Lin ZS et al. Am J Cancer Res 2018; 8 (10):1989-2004 FOXA1 transcriptionally up-regulates cyclin B1 expression to enhance chondrosarcoma progression.
Huang CY et al. Cells 2018; 7 (12) Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins.
Application : WB
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8):4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
Liang HH et al. Life Sci 2018; (Epub) Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation.
Suk FM et al. Sci Rep 2018; 8 (1):2742 ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner.
Application : WB
Reactivity : Human
Luo W et al. World J Surg Oncol 2018; 16 (1):36 The role of kinesin KIF18A in the invasion and metastasis of hepatocellular carcinoma.
Application : WB
Reactivity : Human
Chen HY et al. Int J Mol Sci 2017; 18 (10) Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth.
Application : WB
Reactivity : Human
Yurugi H et al. Oncogene 2017; (Epub) Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.
Application : WB
Reactivity : Human
Yao YF et al. Biochem Pharmacol 2017; (Epub) Identification of novel 1-indolyl acetate-5-nitroimidazole derivatives of combretastatin A-4 as potential tubulin polymerization inhibitors.
Application : WB
Reactivity : Human
Shi CS et al. Anticancer Res 2017; 37 (3):1149-1159 Evodiamine Induces Cell Growth Arrest, Apoptosis and Suppresses Tumorigenesis in Human Urothelial Cell Carcinoma Cells.
Application : WB
Reactivity : Human
Chung TW et al. BMC Complement Altern Med 2017; 17 (1):62 Sinularin induces DNA damage, G2/M phase arrest, and apoptosis in human hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Kuo CY et al. Cell Cycle 2016; 15 (6):787-98 RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner.
Application : WB
Reactivity : Human
Kung YA et al. Nucleic Acids Res 2016; Control of the negative IRES trans-acting factor KHSRP by ubiquitination.
Application : WB
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
Lin YW et al. Neurol Res 2016; 38 (7):625-33 Application of flow cytometry for evaluating clinical prognosis and histopathological grade of human glioma.
Application : WB
Reactivity : Human
So EC et al. Onco Targets Ther 2016; 9:2519-33 Midazolam regulated caspase pathway, endoplasmic reticulum stress, autophagy, and cell cycle to induce apoptosis in MA-10 mouse Leydig tumor cells.
Reactivity : Human
Chin YT et al. Oncotarget 2014; 5 (24):12891-907 Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.
Application : WB
Reactivity : Human
Lin YW et al. Cytometry B Clin Cytom 2014; The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas.
Application : WB
Reactivity : Human
Li LH et al. PLoS One 2014; 9 (8):e104203 hinokitiol induces dna damage and autophagy followed by cell cycle arrest and senescence in *-resistant lung adenocarcinoma cells.
Application : WB
Reactivity : Human
Luo CW et al. Biochim Biophys Acta 2014; 1843 (9):2129-2137 Radiation sensitization of tumor cells induced by shear stress: The roles of integrins and FAK.
Application : WB
Reactivity : Human
Cheung AK et al. Mol Cancer Ther 2013; 12 (8):1393-401 Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Lee YR et al. J Biomed Sci 2012; 19:9 Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagy.
Application : WB
Reactivity : Human
Pan J et al. Oncogene 2010; 29 (47):6245-56 Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Application : WB
Reactivity : Mouse
SDS
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Package List Price ($)
$ 399
$ 169