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Cyclophilin A antibody [N1C3]

KO/KD Orthogonal
Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520
Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520
Anti-Cyclophilin A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113520
Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520
Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520
Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

Cat. No. GTX113520

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cyclophilin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , Raji , 293T

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase A , CYPA , CYPH , HEL-S-69p

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

GTX113520 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [N1C3] (GTX113520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

GTX113520 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [N1C3] (GTX113520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113520

GTX113520 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cyclophilin A(GTX113520) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

GTX113520 WB Image

Orthogonal

Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX113520) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

GTX113520 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113520 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [N1C3] used in Western Blot (WB). GTX113520

GTX113520 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
12% SDS PAGE
GTX113520 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen YH et al. Mol Med Rep 2017; (Epub) H2O2 induces caveolin-1 degradation and impaired mitochondrial function in E11 podocytes.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169