GeneTex
United States (US)

Cytochrome P450 1A1 + 1A2 antibody [MC1]

GTX15616_1021_IHC-P_19011519_953.jpg
GTX15616_1022_IHC-P_19011519_347.jpg
GTX15616_1023_IHC-P_19011519_175.jpg
GTX15616_273_ICC-IF_19011519_840.jpg
GTX15616_271_ICC-IF_19011519_370.jpg
GTX15616_272_ICC-IF_19011519_946.jpg
GTX15616_1020_IHC-P_19011519_723.jpg

Cat No. GTX15616

Host Mouse
Clonality Monoclonal
Clone Name MC1
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

3-methylcholanthrene induced rat cytochrome P450 protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AHH,AHRR,CP11,CYP1,Cyp45c,Cypc45c,P-450MC,P1-450,P450-C,P450DX

Cellular Localization

Cytoplasm,Endoplasmic reticulum,Endoplasmic reticulum membrane,Microsome membrane,Mitochondrion,Mitochondrion membrane

Background

monooxygenase that plays a role in dioxin metabolism and detoxification which is 3-methylcholanthrene-inducible [RGD, Feb 2006]
DATA IMAGES
GTX15616_1021_IHC-P_19011519_953.jpg

GTX15616 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

GTX15616_1022_IHC-P_19011519_347.jpg

GTX15616 IHC-P Image

IHC-P analysis of human prostate tissue using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

GTX15616_1023_IHC-P_19011519_175.jpg

GTX15616 IHC-P Image

IHC-P analysis of rat liver tissue using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX15616_273_ICC-IF_19011519_840.jpg

GTX15616 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:100 incubated overnight at 4 °C

GTX15616_271_ICC-IF_19011519_370.jpg

GTX15616 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:100 incubated overnight at 4 °C

GTX15616_272_ICC-IF_19011519_946.jpg

GTX15616 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:100 incubated overnight at 4 °C

GTX15616_1020_IHC-P_19011519_723.jpg

GTX15616 IHC-P Image

IHC-P analysis of human liver tissue using GTX15616 Cytochrome P450 1A1 + 1A2 antibody [MC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

Package List Price ($)
$ 319