GeneTex
United States (US)

Cytochrome P450 3A4 + 3A5 antibody [F24 P2 B10]

GTX15836_340_ICC-IF_19011519_568.jpg
GTX15836_1547_WB_19011519_465.jpg

Cat No. GTX15836

Host Mouse
Clonality Monoclonal
Clone Name F24 P2 B10
Isotype IgG1
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:250
IHC Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]
DATA IMAGES
GTX15836_340_ICC-IF_19011519_568.jpg

GTX15836 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX15836 Cytochrome P450 3A4 + 3A5 antibody [F24 P2 B10]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

GTX15836_1547_WB_19011519_465.jpg

GTX15836 WB Image

WB analysis of whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4) and Mouse Kidney (Lane 5) using GTX15836 Cytochrome P450 3A4 + 3A5 antibody [F24 P2 B10].
Dilution : 1:100-1:1000

Package List Price ($)
$ 319