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Cytokeratin 13 antibody

Anti-Cytokeratin 13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109883
Anti-Cytokeratin 13 antibody used in Western Blot (WB). GTX109883
Anti-Cytokeratin 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX109883
Anti-Cytokeratin 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX109883
Anti-Cytokeratin 13 antibody used in IHC (Whole mount) (IHC-Wm). GTX109883

Cat. No. GTX109883

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 13 , CK13 , K13 , WSN2

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109883

GTX109883 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13(GTX109883) antibody at 1:200 dilution.

Anti-Cytokeratin 13 antibody used in Western Blot (WB). GTX109883

GTX109883 WB Image

Cytokeratin 13 antibody detects KRT13 protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10% SDS-PAGE
Cytokeratin 13 antibody (GTX109883) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX109883

GTX109883 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13(GTX109883) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX109883

GTX109883 IHC-P Image

Cytokeratin 13 antibody detects Cytokeratin 13 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
Cytokeratin 13 stained by Cytokeratin 13 antibody (GTX109883) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 13 antibody used in IHC (Whole mount) (IHC-Wm). GTX109883

GTX109883 IHC-Wm Image

The data was published in the journal Oncotarget in 2014. PMID: 25051368

REFERENCE

Application Reference

Chen CH et al. Oncotarget 2014; (Epub) Cdc6 cooperates with c-Myc to promote genome instability and EMT in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169