GeneTex

  • Country / Location Selection

United States (US)

Cytokeratin 14 antibody

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124
Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

Cat. No. GTX104124

Reference 14
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950640

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung CA, using Cytokeratin 14(GTX104124) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124

GTX104124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000.

Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124

GTX104124 ICC/IF Image

Cytokeratin 14 antibody detects Cytokeratin 14 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody (GTX104124) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124

GTX104124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

The data was published in the journal Front Oncol in 2013.PMID: 23596564

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

The data was published in the journal Nat Commun in 2019.PMID: 31455775

REFERENCE

Application Reference

Takayasu Moroki et al. J Toxicol Pathol 2021; 34 (2) : 161-180 Databases for technical aspects of immunohistochemistry: 2021 update.
Application : IHC
Reactivity : Rat
Chongfa Tang et al. Sci Rep 2021; 11 (1) : 5848 Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile.
Application : ICC/IF
Reactivity : Human
Kun-Ling Lin et al. Int J Mol Sci 2021; 22 (17) Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial.
Application : WB, ICC/IF
Reactivity : Rat
Kim JY et al. Nat Commun 2019; 10 (1) : 3694 Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy.
Application : IHC-Fr
Reactivity : Human
D'Amico S et al. Genes Dev 2018; 32 (17-18) : 1175-1187 STAT3 is a master regulator of epithelial identity and KRAS-driven tumorigenesis.
Show More +
Submit a Reference

REVIEW

Cytokeratin 14 antibody Cat. No. GTX104124

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Date : Anonymous submitted on 21-Dec-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat Glandula sublimgualis tissue
Blocking : 0.3% H2O2 in methanol, 20min, RT and 2.5% Normal horse seru in TBST, 20min, RT
Fixation : neutral buffered formalin
Permeabilization /Antigen retrieval : TBST/citrate buffer (pH6), MW 10min
Primary Antibody : 1:200, 25°C, 1Hr
Figure Note : Duct epithelial cells and myoepithlial cells are postive. Acinar cells are negative.
Anti-Cytokeratin 14 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104124
Date : Anonymous submitted on 11-Dec-2013
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : olfactory epithelium tissue
Blocking : 5% FBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : citric acid, pH6
Primary Antibody : 1:200, 4°C, 20Hr
Figure Note : IHC analysis of OCT-embedded rat olfactory epithelium,using GTX104124(green).
Submit a Review
Application Reference
Takayasu Moroki et al. J Toxicol Pathol 2021; 34 (2):161-180 Databases for technical aspects of immunohistochemistry: 2021 update.
Application : IHC
Reactivity : Rat
Chongfa Tang et al. Sci Rep 2021; 11 (1):5848 Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile.
Application : ICC/IF
Reactivity : Human
Kun-Ling Lin et al. Int J Mol Sci 2021; 22 (17) Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial.
Application : WB, ICC/IF
Reactivity : Rat
Kim JY et al. Nat Commun 2019; 10 (1):3694 Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy.
Application : IHC-Fr
Reactivity : Human
D'Amico S et al. Genes Dev 2018; 32 (17-18):1175-1187 STAT3 is a master regulator of epithelial identity and KRAS-driven tumorigenesis.
Wang S et al. Med Sci (Paris) 2018; 34 Focus issue F1:94-98 Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Application : IHC-Fr
Reactivity : Human
Lee YL et al. Am J Pathol 2017; 187 (9):1945-1959 elucidating mechanisms of bladder repair after hyaluronan instillation in *-induced ulcerative cystitis in animal model.
Application : WB, IHC
Reactivity : Rat
Dang NN et al. Braz J Med Biol Res 2015; 48 (1):39-45 Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Shen CH et al. Mol Med Rep 2015; 11 (2):887-95 biological effect of * in urothelial cell lines and global gene expression analysis in the bladders of *-injected mice.
Application : IHC
Reactivity : Human
Igawa K et al. Stem Cells Transl Med 2014; 3 (9):992-1001 Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
Application : ICC/IF
Reactivity : Human
Dang N et al. Mol Biol Rep 2014; Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
Wang S et al. J Biol Chem 2013; 288 (36):26265-74 Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
Application : WB
Reactivity : Human
Hassiotou F et al. Front Oncol 2013; 3:79 Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169