Cytokeratin 18 antibody [1E1]

Cat. No. GTX84256

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	1E1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Monkey
Package	100 μl ($519)

See all Cytokeratin 18 products

View product citations for antibody GTX84256 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10726718

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Nucleolus

Background

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX84256 WB Image

WB analysis of HEK293T cells transfected with Cytokeratin 18 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 5 ug per lane

GTX84256 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120ººC for 3 min.
Dilution : 1:6000

GTX84256 IHC-P Image

IHC-P analysis of human liver tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

GTX84256 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 WB Image

WB analysis of various cell lines using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 35 ug per lane

GTX84256 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 10 ug per lane
Dilution : 1:200

GTX84256 IHC-P Image

IHC-P analysis of human colon tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 WB Image

WB analysis of HepG2 cell lysate using GTX84256 Cytokeratin 18 antibody [1E1].
Dilution : 1:1000

GTX84256 IHC-P Image

IHC-P analysis of human breast tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 18 plasmid using GTX84256 Cytokeratin 18 antibody [1E1].

GTX84256 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84256 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

REFERENCE

Application Reference

Tong J et al. PLoS One 2018; 13 (10) : e0204077 Adipose-derived mesenchymal stem cells formed acinar-like structure when stimulated with breast epithelial cells in three-dimensional culture.

Application : ICC/IF

Reactivity : Human

Submit a Reference

REVIEW

There are currently no reviews for Cytokeratin 18 antibody [1E1] (GTX84256). Be the first to share your experience with this product.

Submit a Review

Application Reference

Tong J et al. PLoS One 2018; 13 (10):e0204077 Adipose-derived mesenchymal stem cells formed acinar-like structure when stimulated with breast epithelial cells in three-dimensional culture.

Application : ICC/IF

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μl	$ 519
Qty

Cytokeratin 18 antibody [1E1]

Cat. No. GTX84256

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84256 WB Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 WB Image

GTX84256 WB Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 WB Image

GTX84256 IHC-P Image

GTX84256 ICC/IF Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

GTX84256 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.