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Cytokeratin 8 (phospho Ser74) antibody

Orthogonal
Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320
Anti-Cytokeratin 8 (phospho Ser74) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50320
Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320

Cat. No. GTX50320

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from human Cytokeratin 8.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320

GTX50320 WB Image

Orthogonal

WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50320

GTX50320 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320

GTX50320 WB Image

Orthogonal

WB analysis of extracts from 293 cells treated with calf intestinal phosphatase (CIP) using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399