APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DAG1 / beta Dystroglycan. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885601
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9
Cellular Localization
Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton
Background
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225](/templates/default/js/lightbox/images/loading.gif "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225")
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GTX124225 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [C2C3-2], C-term (GTX124225) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX124225 ICC/IF Image
beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta dystroglycan protein stained by beta dystroglycan antibody [C2C3-2], C-term (GTX124225) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX124225 IHC-P Image
beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
beta dystroglycan antibody [C2C3-2], C-term (GTX124225) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
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![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225](/upload/website/prouct_img/small/GTX124225/GTX124225_43384_20181109_WB_w_23060522_995.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225")
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225](/upload/website/prouct_img/small/GTX124225/GTX124225_40961_IFA_w_23060522_893.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225")
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225](/upload/website/prouct_img/small/GTX124225/GTX124225_40961_IHC_M_w_23060522_360.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225")
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225](/upload/website/prouct_img/normal/GTX124225/GTX124225_43384_20181109_WB_w_23060522_995.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225")
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225](/upload/website/prouct_img/normal/GTX124225/GTX124225_40961_IFA_w_23060522_893.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225")
![Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225](/upload/website/prouct_img/normal/GTX124225/GTX124225_40961_IHC_M_w_23060522_360.webp "Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225")