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DAK antibody

Anti-DAK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103102
Anti-DAK antibody used in Western Blot (WB). GTX103102
Anti-DAK antibody used in IHC (Paraffin sections) (IHC-P). GTX103102

Cat. No. GTX103102

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950089

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

triokinase and FMN cyclase , DAK , NET45

Background

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103102

GTX103102 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DAK(GTX103102) antibody at 1:500 dilution.

Anti-DAK antibody used in Western Blot (WB). GTX103102

GTX103102 WB Image

Sample(30 μg whole cell lysate)
A:Hep G2(GTX27900)
B:Raji(GTX27908)
7.5% SDS PAGE
GTX103102 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DAK antibody used in IHC (Paraffin sections) (IHC-P). GTX103102

GTX103102 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using DAK(GTX103102) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169