APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Lysate(GTX27918) , Rat Brain Tissue Lysate(GTX27942)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK1.The immunogen is located within the last 50 amino acids of DCLK1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
doublecortin like kinase 1 , CL1 , CLICK1 , DCAMKL1 , DCDC3A , DCLK
Background
DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX- mice.
Database
Research Area
DATA IMAGES
|
GTX85008 WB Image
WB analysis of rat brain tissue lysate using GTX85008 DCLK1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
|
|
|
GTX85008 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85008 DCLK1 antibody.
Working concentration : 20 μg/ml
|
|
|
GTX85008 WB Image
WB analysis of human brain tissue lysate using GTX85008 DCLK1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
|
|
|
GTX85008 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85008 DCLK1 antibody.
Working concentration : 5 μg/ml
|
|
|
GTX85008 IHC-P Image
IHC-P analysis of rat brain tissue using GTX85008 DCLK1 antibody.
Working concentration : 2.5 μg/ml
|
REFERENCE
There are currently no references for DCLK1 antibody (GTX85008). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DCLK1 antibody (GTX85008). Be the first to share your experience with this product.
