Reconstitute in ddH2O. Store at -20ºC.
Purity was assessed by SDS-PAGE (≥99%) and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Chromodomain Helicase Dna Binding Protein 7,Crg,Hh5,Is3,Kal5,Chd7
DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete beta-barrel capped at the opening by a C-terminal alpha -helix; they bind to the minor groove of a DNA duplex via the triple-stranded beta-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. in vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.