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DNMT3A antibody

KO/KD Orthogonal
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129125
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129125
Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129125
Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

Cat. No. GTX129125

Reference 13
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, EM

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNMT3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
EM Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

Neuro2A , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus , Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

KO/KD

Wild-type (WT) and DNMT3A knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129125

GTX129125 IP Image

DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments.
IP samples: 293T nuclear extract
A. 50 μg 293T nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of DNMT3A protein by 4 μg DNMT3A antibody (GTX129125)
5 % SDS-PAGE
The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129125) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129125

GTX129125 IHC-P Image

DNMT3A antibody detects DNMT3A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
DNMT3A stained by DNMT3A antibody (GTX129125) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129125

GTX129125 IHC-P Image

DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
DNMT3A antibody (GTX129125) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129125

GTX129125 IP Image

Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 μg of DNMT3A antibody (GTX129125) or DNMT3A antibody (GTX129126).
Western blot analysis was performed using DNMT3A antibody (GTX129125) diluted at 1:2000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129125

GTX129125 ICC/IF Image

DNMT3A antibody detects DNMT3A protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3A stained by DNMT3A antibody (GTX129125) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2018. PMID: 29416619

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2018. PMID: 29416619

Anti-DNMT3A antibody used in Western Blot (WB). GTX129125

GTX129125 WB Image

The data was published in the journal PLoS One in 2015. PMID: 25928539

REFERENCE

Application Reference

L Zhang et al. Pharmacogn Mag 2024; RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Wu YH et al. J Ovarian Res 2023; 16 (1) : 124 The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells.
Wu YH et al. Res Sq 2023; The Downregulation of miR-509-3p Expression by Collagen Type XI Alpha 1-Regulated Hypermethylation Facilitates Cancer Progression and Chemoresistance via the DNA Methyltransferase 1/Small Ubiquitin-like Modifier-3 Axis in Ovarian Cancer Cells.
Williamson SM et al. J Insect Physiol 2021; 134 : 104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
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Submit a Reference

REVIEW

DNMT3A antibody Cat. No. GTX129125

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Electron microscopy(EM)
( Average 4 based on 1 users reviews)
Anti-DNMT3A antibody used in Electron microscopy (EM). GTX129125
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:5, 4°C, 16Hr
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Date : Anonymous submitted on 07-Jun-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : NIH/3T3 cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-DNMT3A antibody used in Western Blot (WB). GTX129125
Date : Anonymous submitted on 26-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-0 cells
Amount used : 50μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 2Hr
Submit a Review
Application Reference
L Zhang et al. Pharmacogn Mag 2024; RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Wu YH et al. J Ovarian Res 2023; 16 (1):124 The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells.
Wu YH et al. Res Sq 2023; The Downregulation of miR-509-3p Expression by Collagen Type XI Alpha 1-Regulated Hypermethylation Facilitates Cancer Progression and Chemoresistance via the DNA Methyltransferase 1/Small Ubiquitin-like Modifier-3 Axis in Ovarian Cancer Cells.
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Yan JB et al. Mol Ther Oncolytics 2020; 18:282-294
Liao CH et al. Mar Drugs 2019; 17 (9) Epigenetic Modification and Differentiation Induction of Malignant Glioma Cells by Oligo-Fucoidan.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Alessandrini F et al. Neoplasia 2018; 20 (8):857-870 ETV7-Mediated DNAJC15 Repression Leads to Doxorubicin Resistance in Breast Cancer Cells.
Huang MH et al. Pharmacol Res 2018; 139:50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1):361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Zhang Q et al. Sci Rep 2016; 6:34597 Rhein reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal fibrosis in mice.
Application : WB
Reactivity : Mouse
Wen HC et al. PLoS One 2015; 10 (4):e0125518 Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169