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Deltex1 antibody [C1C3]

Anti-Deltex1 antibody [C1C3] used in Western Blot (WB). GTX112367
Anti-Deltex1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112367
Anti-Deltex1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112367

Cat. No. GTX112367

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Deltex1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Deltex1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

deltex E3 ubiquitin ligase 1 , RNF140 , hDx-1

Cellular Localization

Cytoplasm , Nucleus

Background

Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Deltex1 antibody [C1C3] used in Western Blot (WB). GTX112367

GTX112367 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112367 diluted at 1:1000

Anti-Deltex1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112367

GTX112367 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Deltex1(GTX112367) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Deltex1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112367

GTX112367 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Deltex1(GTX112367) antibody at 1:200 dilution.

REFERENCE

Application Reference

Hou G et al. Onco Targets Ther 2019; 12 : 5215-5225 LSD1 regulates Notch and PI3K/Akt/mTOR pathways through binding the promoter regions of Notch target genes in esophageal squamous cell carcinoma.
Application : WB
Reactivity : Human
ZhaomingLu et al. Biomedicine & Pharmacotherapy 2018; 107 : 1370-1376 FLI-06 suppresses proliferation, induces apoptosis and cell cycle arrest by targeting LSD1 and Notch pathway in esophageal squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169