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Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)]

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

Cat No. GTX29202

Host Mouse
Clonality Monoclonal
Clone Name D1-11(3)
Isotype IgG2a
Application WB, ICC/IF, Dot, ELISA
Reactivity Dengue virus
Package
250 μg ($319)
APPLICATION

Application Note

For Dot: Use at an assay dependent dilution. For ELISA: Use at an assay dependent dilution. For WB: Use at a concentratio of 1.0 μg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

This hybridoma antibody reacts with each dengue serotype in dot blot, ELISA and fluorescent antibody tests. Its reactivity has been confirmed by CDC.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Mice were immunized with a mixture of dengue 1, 2, 3 and 4. Spleen cells were used to prepare hybridomas and clones selected based on the ability of the antibody they produced to react with each dengue serotype.

Purification

Protein A purified
From hybridoma culture supernatant.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

GTX29202 ICC/IF Image

Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 1 protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: Dengue virus 1 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] (GTX29202) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

GTX29202 ICC/IF Image

Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 2 protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Dengue virus 2 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] (GTX29202) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

GTX29202 ICC/IF Image

Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 4 protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: Dengue virus 4 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] (GTX29202) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

GTX29202 ICC/IF Image

Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 3 protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Dengue virus 3 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] (GTX29202) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] Cat No. GTX29202

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-Jul-2014
Score :
Application Tested : WB
Sample Species : DENV
Sample : DV infected vero cells
Amount used : 1x10^6 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Figure Note : starreivew courtesy of Guey-Chuen Perng, Ph.D. (Laboratory of National Cheng Kung University)
Application Reference
Package List Price ($)
$ 319