GeneTex

  • Country / Location Selection

United States (US)

Dengue virus prM protein antibody

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

Cat. No. GTX128092

Reference 13
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus prM protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

Product Note

This antibody was raised against Dengue virus 2 prM protein, which may cross react with prM protein of Dengue virus 1/3/4.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus PrM protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Premembrane , Dengue Virus PrM , DENV Premembrane , DENV PrM

Research Area

DATA IMAGES

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128092) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

Non-infected (–) and infected (+) Zebrafish embryos extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus prM protein antibody (GTX128092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25122182

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

The data was published in the journal Front Microbiol in 2019. PMID: 31824450

REFERENCE

Application Reference

Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Pan P et al. Front Microbiol 2019; 10 : 2637 Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage.
Pan P et al. J Virol 2019; (Epub) Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation to Induce Vascular Leakage in Mice.
Application : WB
Utomo DIS et al. Mol Biotechnol 2019; (Epub) Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
Application : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
Application : WB
Show More +
Submit a Reference

REVIEW

Dengue virus prM protein antibody Cat. No. GTX128092

Rating
( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 3.5 based on 2 users reviews)
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Date : Anonymous submitted on 25-Dec-2019
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Huh7 whole cell lysate; Lane 2: Dengue virus type II NGC strain infected.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Dengue virus type 2 infected BHK21 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1 and 2: BHK-21 cells. Lane 3 and 4: Dengue virus type 2 (PLO46) infected BHK-21 cells.
Submit a Review
Application Reference
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Pan P et al. Front Microbiol 2019; 10:2637 Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage.
Pan P et al. J Virol 2019; (Epub) Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation to Induce Vascular Leakage in Mice.
Application : WB
Utomo DIS et al. Mol Biotechnol 2019; (Epub) Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
Application : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
Application : WB
Puschnik AS et al. Cell Rep 2017; 21 (11):3032-3039 A Small-Molecule Oligosaccharyltransferase Inhibitor with Pan-flaviviral Activity.
Application : WB
Chen M et al. Sci Rep 2017; 7 (1):15931 Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane.
Lin C et al. Antiviral Res 2017; (Epub) Design, synthesis, and biological evaluation of novel 7-deazapurine nucleoside derivatives as potential anti-dengue virus agents.
Application : WB
Soto-Acosta R et al. PLoS Pathog 2017; 13 (4):e1006257 DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target.
Application : WB
Mukherjee S et al. Virology 2016; 497:33-40 Enhancing dengue virus maturation using a stable furin over-expressing cell line.
Application : WB
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Lee CM et al. J Virol 2015; 89 (12):6171-83 Determinants of Dengue Virus NS4A Protein Oligomerization.
Application : WB
Zhu X et al. PLoS Negl Trop Dis 2014; 8 (8):e3088 microrna-30e* suppresses dengue virus replication by promoting nf-κb-dependent ifn production.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169