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EAAT4 antibody

Anti-EAAT4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134100
Anti-EAAT4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134100
Anti-EAAT4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134100

Cat. No. GTX134100

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EAAT4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2847827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 , EAAT4

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-EAAT4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134100

GTX134100 ICC/IF Image

EAAT4 antibody detects EAAT4 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EAAT4 stained by EAAT4 antibody (GTX134100) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EAAT4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134100

GTX134100 IHC-Fr Image

EAAT4 antibody detects EAAT4 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: EAAT4 stained by EAAT4 antibody (GTX134100) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EAAT4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134100

GTX134100 IHC-P Image

EAAT4 antibody detects EAAT4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EAAT4 stained by EAAT4 antibody (GTX134100) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169