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ECH1 antibody [9D11AF3]

Anti-ECH1 antibody [9D11AF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54746

Cat. No. GTX54746

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9D11AF3

Isotype

 IgG1

Application

 ICC/IF, IP, ELISA

Reactivity

 Human, Rat
Package
100 μg ($399)
See all ECH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 4 μg/ml
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

HEPES buffered saline

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to the peroxisome

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA hydratase 1 , HPXEL

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ECH1 antibody [9D11AF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54746

GTX54746 ICC/IF Image

ICC/IF analysis of HDFn cells using GTX54746 ECH1 antibody [9D11AF3].
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde 20 mins
Permeabilization : Triton X-100 (0.1%, 15 min)
Dilution : 4 μg/ml overnight at 4ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399