GeneTex
  • Country / Location Selection

United States (US)

ECHS1 antibody [N1C2]

Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

Cat No. GTX114376

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enoyl-Coa Hydratase, Short Chain 1,Echs1D,Sceh,Echs1

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376

GTX114376 IP Image

Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] (GTX114376).
Western blot analysis was performed using ECHS1 antibody [N1C2] (GTX114376).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114376 diluted at 1:1000

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW
There are currently no reviews for ECHS1 antibody [N1C2] (GTX114376). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169