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EI24 antibody

Anti-EI24 antibody used in IHC (Paraffin sections) (IHC-P). GTX31841
Anti-EI24 antibody used in Western Blot (WB). GTX31841

Cat. No. GTX31841

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all EI24 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Rat Liver Tissue Lysate(GTX27943)

Product Note

At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

EI24 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human EI24.The immunogen is located within the last 50 amino acids of EI24.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EI24 autophagy associated transmembrane protein , EPG4 , PIG8 , TP53I8

Cellular Localization

Cytoplasm,Endoplasmic reticulum membrane

Background

This gene has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The protein encoded by this gene contains six putative transmembrane domains and may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EI24 antibody used in IHC (Paraffin sections) (IHC-P). GTX31841

GTX31841 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31841 EI24 antibody.
Working concentration : 5 μg/ml

Anti-EI24 antibody used in Western Blot (WB). GTX31841

GTX31841 WB Image

WB analysis of rat liver tissue lysate using GTX31841 EI24 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399