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EMI1 antibody, C-term

Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926
Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926
Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926

Cat. No. GTX46926

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($399)
See all EMI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HepG2 , human fetal lung , human placenta

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human EMI1.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box protein 5 , EMI1 , FBX5 , Fbxo31

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926

GTX46926 WB Image

WB analysis of HepG2 cells using GTX46926 FBXO5 antibody at 0.2-1μg/ml.

Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926

GTX46926 WB Image

WB analysis of human placenta tissue using GTX46926 FBXO5 antibody at 1.0μg/ml.

Anti-EMI1 antibody, C-term used in Western Blot (WB). GTX46926

GTX46926 WB Image

WB analysis of human fetal lung tissue using GTX46926 FBXO5 antibody at 1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399