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ENPP1 antibody [N2C2], Internal

KO/KD Overexpression
Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447
Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447
Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447
Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447

Cat. No. GTX103447

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ENPP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

HepG2 , human liver

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ENPP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730638

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ectonucleotide pyrophosphatase/phosphodiesterase 1 , ARHR2 , COLED , M6S1 , NPP1 , NPPS , PC-1 , PCA1 , PDNP1

Background

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447

GTX103447 WB Image

ENPP1 antibody detects ENPP1 protein by Western blot analysis. Human tissue extracts (30 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with ENPP1 antibody (GTX103447) at a dilution of 1:1000.

Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447

GTX103447 WB Image

ENPP1 antibody detects ENPP1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ENPP1 antibody (GTX103447) at a dilution of 1:500.

Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447

GTX103447 WB Image

KO/KD Overexpression

The data was published in the journal Nat Commun in 2017.PMID: 28281525

Anti-ENPP1 antibody [N2C2], Internal used in Western Blot (WB). GTX103447

GTX103447 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28281525

REFERENCE

Application Reference

Benjamin M Adams et al. Elife 2020; 9 Quantitative glycoproteomics reveals cellular substrate selectivity of the ER protein quality control sensors UGGT1 and UGGT2.
Application : WB
Reactivity : Human
Huang CK et al. Nat Commun 2017; 8 : 14706 Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169