GeneTex

  • Country / Location Selection

United States (US)

EP300 antibody [RW109]

Anti-EP300 antibody [RW109] used in Western Blot (WB). GTX30619

Cat. No. GTX30619

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RW109

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Mustelid, Primate
Package
200 μl ($429)
See all EP300 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100 - 1:200
IP 1:10 - 1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

Product Note

This is specific for p300 protein. This recognizes residues 2107-2283.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.

Database

Research Area

DATA IMAGES

Anti-EP300 antibody [RW109] used in Western Blot (WB). GTX30619

GTX30619 WB Image

WB analysis of HeLa nuclear extract using GTX30619 EP300 antibody [RW109].
Dilution : 1:1000

REFERENCE

Application Reference

Hsing-Hua Chao, Jun-Liang Luo et al. Cell Death Dis . 2022; 13 (9) Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells
Application : ChIP assay
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for EP300 antibody [RW109] (GTX30619). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 429