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ER81 antibody

Orthogonal Overexpression
Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202
Anti-ER81 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129202
Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202
Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202
Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202
Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

Cat. No. GTX129202

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ER81 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Rat2

Product Note

This antibody was rasied against ER81 isoform 1. Based on sequence homology, this antibody can recognize most ER81 isoforms, but it may be unable to recognize isoform 4 according to the customer's feedback.

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ER81. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2797598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS variant 1 , ER81

Cellular Localization

Nucleus

Background

This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202

GTX129202 IHC-P Image

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202

GTX129202 IHC-P Image

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ER81 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129202

GTX129202 IHC-Fr Image

ER81 antibody detects ER81 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

GTX129202 ICC/IF Image

ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis.
Sample: C8D30 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 protein stained by ER81 antibody (GTX129202) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202

GTX129202 IHC-P Image

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

GTX129202 ICC/IF Image

ER81 antibody detects ER81 protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-ER81 antibody used in IHC (Paraffin sections) (IHC-P). GTX129202

GTX129202 IHC-P Image

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Orthogonal

U87-MG whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

GTX129202 ICC/IF Image

ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Batzorig U et al. Int J Med Sci 2021; 18 (11) : 2251-2261 Glucose-Regulated Protein 94 Mediates the Proliferation and Metastasis through the Regulation of ETV1 and MAPK Pathway in Colorectal Cancer.
Application : WB
Reactivity : Human
Bordignon P et al. Cell Rep 2019; 28 (9) : 2358-2372.e6 Dualism of FGF and TGF-帣 Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169